Journal: Journal of Clinical Microbiology

Article Title: Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures

doi: 10.1128/JCM.00485-18

Figure Lengend Snippet: Comparative performance of iC-GPC Assay with Verigene method and resolution of discordant test results following PCR/sequencing arbitration testing

Article Snippet: This study comparatively evaluated the performance of the iC-GPC Assay against the Verigene Gram-positive blood culture (BC-GP) assay (Luminex Corp., Austin, TX) for 1,134 patient blood culture specimens positive for Gram-positive cocci.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures

doi: 10.1128/JCM.00485-18

Figure Lengend Snippet: Comparative performances of mixed-culture specimens

Article Snippet: This study comparatively evaluated the performance of the iC-GPC Assay against the Verigene Gram-positive blood culture (BC-GP) assay (Luminex Corp., Austin, TX) for 1,134 patient blood culture specimens positive for Gram-positive cocci.

Techniques:

Journal: ACS chemical biology

Article Title: A gut-restricted lithocholic acid analog as an inhibitor of gut bacterial bile salt hydrolases

doi: 10.1021/acschembio.1c00192

Figure Lengend Snippet: a, Assay design for screening the inhibitor library. Screening in fresh mouse feces identified AAA-10 as a potent second-generation pan-inhibitor of BSHs. Inhibitors were tested at a concentration of 10 μM. Sulfonation did not reduce the potency of AAA-10 compared to AAA-9. Assay was performed three times independently with similar results. Figure shows representative results from one assay. b and c, AAA-10 is more potent than AAA-2 against recombinant BSHs. Comparison of AAA-10 and AAA-2 dose-response curves against Bacteroides thetaiotaomicron (B. theta, accession number AA077193.1) and Bifidobacterium longum (B. longum, accession number AAF67801.1) BSHs using tauro-ursodeoxycholic acid (TUDCA) and tauro-deoxycholic acid (TDCA) as the respective substrates. See Table S1 for comparison of IC50 values of AAA-1, AAA-2 and AAA-10. For b and c, Graphpad was used to fit IC50 curves. All assays were performed in biological triplicate, and data are presented as mean ± s.e.m.

Article Snippet: The BSH inhibitory activity of AAA-10 (100 μM) against three Gram-negative ( B. theta VPI-5482, Bacteroides fragilis ATCC 25285, and Bacteroides vulgatus ATCC 8482) and three Gram-positive ( Lactobacillus plantarum WCFS1, Clostridium perfringens ATCC 13124, and Bifidobacterium adolescentis L2–32) human gut bacteria using 100 μM taurine-conjugated bile acids (TβMCA, TCA, TUDCA and TDCA, 25 μM each) as substrates was evaluated.

Techniques: Library Screening, Concentration Assay, Recombinant, Comparison

Journal: ACS chemical biology

Article Title: A gut-restricted lithocholic acid analog as an inhibitor of gut bacterial bile salt hydrolases

doi: 10.1021/acschembio.1c00192

Figure Lengend Snippet: a, AAA-10 inhibits bacterial BSH activity. The BSH inhibitory activity of AAA-10 (100 μM) against three Gram-negative (B. theta VPI-5482, Bacteroides fragilis ATCC 25285, and Bacteroides vulgatus ATCC 8482) and three Gram-positive (Lactobacillus plantarum WCFS1, Clostridium perfringens ATCC 13124, and Bifidobacterium adolescentis L2–32) human gut bacteria using 100 μM taurine-conjugated bile acids (TβMCA, TCA, TUDCA and TDCA, 25 μM each) as substrates was evaluated. BSH activity was quantified as percent deconjugation of tauro-conjugated bile acids at 24 h as determined by UPLC–MS (for absolute concentrations of substrates and products recovered, see Figure S2). b, AAA-10 did not affect bacterial cell viability. At the end of the assay in (a), the bacteria were plated to determine cell viability. c, AAA-10 is a nanomolar inhibitor of bacterial BSHs. Dose-response curves of AAA-10 against B. theta and B. adolescentis cultures were generated using tauro-ursodeoxycholic acid (TUDCA) and tauro-deoxycholic acid (TDCA) as substrates, respectively. For a, and b, two-tailed Student’s t-test were performed. For c, Graphpad was used to fit IC50 curves. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns = not significant. All assays were performed in biological triplicate, and data are presented as mean ± s.e.m.

Article Snippet: The BSH inhibitory activity of AAA-10 (100 μM) against three Gram-negative ( B. theta VPI-5482, Bacteroides fragilis ATCC 25285, and Bacteroides vulgatus ATCC 8482) and three Gram-positive ( Lactobacillus plantarum WCFS1, Clostridium perfringens ATCC 13124, and Bifidobacterium adolescentis L2–32) human gut bacteria using 100 μM taurine-conjugated bile acids (TβMCA, TCA, TUDCA and TDCA, 25 μM each) as substrates was evaluated.

Techniques: Activity Assay, Bacteria, Generated, Two Tailed Test